Journal: PLoS ONE

Article Title: The GDNF System Is Altered in Diverticular Disease – Implications for Pathogenesis

doi: 10.1371/journal.pone.0066290

Figure Lengend Snippet: GDNF increases mRNA expression of synaptophysin in rat enteric nerve cell cultures. Expression levels were measured after one week and are normalized to expression of the house-keeping gene HPRT. Data are shown as mean +/− SEM, n = 11–12 per experimental group, *p<0.05 vs. control.

Article Snippet: After fixation and permeabilization as described above, samples were incubated with a rabbit anti-synaptophysin antibody (1∶1000, Biozol, Echingen, Germany) and a mouse anti-PGP 9.5 antibody (1∶1000, Acris, Herford, Germany) for 1 h. After 30 min incubation with the secondary antibodies, goat anti-rabbit-AlexaFlour488 (1∶250, Invitrogen, Karlsruhe, Germany) and goat-anti-mouse-AlexaFluor546 (1∶250, Invitrogen, Karlsruhe, Germany), cells were counterstained with DAPI (Roche, Mannheim, Germany) to visualize cell nuclei.

Techniques: Expressing, Control

Journal: PLoS ONE

Article Title: The GDNF System Is Altered in Diverticular Disease – Implications for Pathogenesis

doi: 10.1371/journal.pone.0066290

Figure Lengend Snippet: Rat enteric nerve cells were cultured for 3 week without (A-C) or with 50 ng/ml GDNF (D-F). Dual label immunocytochemistry for synaptophysin (green, A, D) and the pan-neuronal marker PGP 9.5 (B, E) was performed. In the merged pictures (C, F) cellular nuclei are stained with DAPI (blue). Cell cultures treated with GDNF display punctuate and granular synaptophysin immunoreactivity along ramifying nerve fibers most likely resembling accumulated synaptic vesicles, whereas in untreated cell cultures immunoreactive signals were confined to neuronal somata. Magnification: 40×.

Article Snippet: After fixation and permeabilization as described above, samples were incubated with a rabbit anti-synaptophysin antibody (1∶1000, Biozol, Echingen, Germany) and a mouse anti-PGP 9.5 antibody (1∶1000, Acris, Herford, Germany) for 1 h. After 30 min incubation with the secondary antibodies, goat anti-rabbit-AlexaFlour488 (1∶250, Invitrogen, Karlsruhe, Germany) and goat-anti-mouse-AlexaFluor546 (1∶250, Invitrogen, Karlsruhe, Germany), cells were counterstained with DAPI (Roche, Mannheim, Germany) to visualize cell nuclei.

Techniques: Cell Culture, Immunocytochemistry, Marker, Staining

Journal: The Journal of Neuroscience

Article Title: Glutamate Transporter GLAST Is Expressed in the Radial Glia–Astrocyte Lineage of Developing Mouse Spinal Cord

doi: 10.1523/JNEUROSCI.17-23-09212.1997

Figure Lengend Snippet: Double-labeling showing morphological and immunochemical properties of GLAST-expressing cells at E13 (A, B), E15 (C, D), and P7 (E–G). A, GLAST immunoreactivity (green) and nuclear staining with propidium iodide (red). B–D, GLAST immunoreactivity (green) and nestin immunoreactivity (red) in the marginal zone (B, D) and mantle zone (C).Arrowheads indicate GLAST-immunopositive punctate or irregular protrusions from radial processes. Arrowsindicate conical endfeet of radial processes. E, GLAST mRNA (purple) and GFAP immunoreactivity (brown). Arrows indicate cell bodies labeled for the GLAST mRNA. F, GLAST immunoreactivity (green) and GFAP immunoreactivity (red). G, GLAST immunoreactivity (green) and synaptophysin immunoreactivity (red). Mg, Marginal zone;Mn, mantle zone; N, neuronal cell body;P, pial surface; Vn, ventricular zone; *, central canal. Scale bars, A–D, F, 10 μm; E, 50 μm; G, 5 μm.

Article Snippet: For double-labeling immunofluorescence, paraffin sections were incubated overnight at room temperature with guinea pig anti-GLAST antibody (2.5 μg/ml) and with rabbit anti-nestin antibody (1:500) ( Tomooka et al., 1993 ), rabbit anti-GFAP antibody (1:1), or rabbit anti-synaptophysin antibody (1:200) (Biotechnik, Heidelberg, Germany), respectively.

Techniques: Labeling, Expressing, Staining

Journal: The Journal of Neuroscience

Article Title: Neuropathic Pain Memory Is Maintained by Rac1-Regulated Dendritic Spine Remodeling after Spinal Cord Injury

doi: 10.1523/JNEUROSCI.3142-08.2008

Figure Lengend Snippet: Western blot analysis of synapse-associated proteins in lumbar spinal cord tissue from intact, SCI and control, vehicle- or NSC23766-treated animals. Quantification was performed on three blots for each protein. To normalize for variation between blots, β-actin control protein level was set to 100% in each lane and proteins of interest were compared across treatment groups. A, Phosphorylated Rac1 protein was probed in the three treatment groups with a visible band at ∼21 kDa. B, Significantly increased levels of phosphorylated Rac1 were found after SCI plus veh treatment compared with uninjured controls. Post-SCI levels were reduced with intrathecal NSC23766. C, D, No significant difference in the levels of total Rac (∼21 kDa) was observed between the treatment groups. E, F, PSD-95 band is visible at ∼95 kDa (E), and significantly higher levels were observed in SCI plus veh compared with intact levels (F). NSC23766 treatment also significantly reduced PSD-95 levels below that of SCI plus veh and intact animals. G, A cortactin band was observed at ∼80 kDa along with breakdown products. H, No differences were observed for levels of cortactin between groups. I, J, We also observed a trend, which did not reach statistical significance, toward an increase in the levels of synaptophysin after SCI. Graphs are mean ± SEM. *p < 0.05; **p < 0.01.

Article Snippet: The following primary antibodies were used: rabbit anti-phospho Rac1 plus Cdc42 (Abcam; 1:250), mouse anti-Rac1 (Millipore), rabbit anti-cortactin (Santa Cruz Biotechnologies; 1:2000), mouse anti-PSD-95 (Abcam; 1:2000), rabbit anti-synaptophysin (GeneTex), and rabbit anti-β-actin (Abcam; 1:5000).

Techniques: Western Blot

Journal: Oncology Letters

Article Title: Next generation DNA sequencing of atypical choroid plexus papilloma of brain: Identification of novel mutations in a female patient by Ion Proton

doi: 10.3892/ol.2019.10882

Figure Lengend Snippet: (A) Immunohistochemistry staining was strongly positive for Ki-67 in atypical CPP (magnification, 10×). (B) Microphotograph demonstrated a strong cytoplasmic immunopositivity for S-100 in the fibrovascular papillae lined by monomorphic epithelial cells (magnification, 10×). (C) Immunohistochemistry staining for synaptophysin was focally positive (magnification, 10×).

Article Snippet: CONFIRM anti-Synaptophysin (SP11) rabbit monoclonal antibody (1:20; cat. no. 790-4407), CONFIRM anti-S100 (1:50; rabbit polyclonal; cat. no. 760-2523), mouse monoclonal β-catenin (1:50; cat. no. 224M-1; Sigma-Aldrich; Merck KGaA), E-cadherin mouse monoclonal (1:50; cat. no. 790-4497; Ventana Medical Systems, Inc.) and mouse monoclonal anti-Ki-67 (1:20; cat. no. KI67-MM1-L-CE; Leica Microsystems, Inc.) antibodies were used for immunohistochemistry.

Techniques: Immunohistochemistry, Staining

Journal: Chinese Journal of Lung Cancer

Article Title: 神经内分泌分化不是非小细胞肺癌高恶性度的指标

doi: 10.3779/j.issn.1009-3419.2011.08.03

Figure Lengend Snippet: 主要试剂、来源及工作浓度 Main reagents, sources and working concentrations

Article Snippet: Rabbit anti-human synaptophysin (Syn) monoclonal antibody , ZSGB-BIO , SP11 , 1:100.

Techniques: